Paper-based Microfluidic Electrochemical Immunodevices Integrated with Nanobioprobes on Graphene Film for Ultrasensitive Detection of Cancer Biomarkers
نویسندگان
چکیده
ABSTRACT We report a novel signal amplification strategy integrated into a microfluidic paper-based electrochemical immunodevice to perform multiplexed measurement of cancer biomarkers. The signal amplification was achieved by using graphene to modify the immunodevice surface to accelerate electron transfer and silica nanoparticle as a tracing tag to label signal antibodies. Accurate, rapid, simple and inexpensive point-of-care electrochemical immunoassays were demonstrated with a photoresist-patterned microfluidic paper-based analytical device. Using the horseradish peroxidase (HRP)-O-phenylenediamine-H2O2 electrochemical detection system, the potential clinical applicability of this device was demonstrated through its ability to identify four candidate cancer biomarkers in serum samples from cancer patients.
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